CREATION OF NEW L-THREONINE-PRODUCING BACTERIUM FROM ESHERRICHIA COLI AND PRODUCTION OF L-THREONINE BY THE SAME BACTERIUM

摘要

PURPOSE: To provide the subject new recombinant plasmid DNA capable of creating a new threonine-mass-producing microorganism by transforming Bacillus coli and growing the transformant in a culture medium containing very low-cost molasses over many generations. ;CONSTITUTION: The objective recombinant plasmid DNA is obtained by integrating a threonine operon, i.e., threonine biosynthesis-related gene in which the attenuation region as a transcription-controlling region is missing into the downstream of the temperature sensitive C₁ repressor, the PR promoter and the C_ro protein-N terminal of a lambda phage and structured so that manifestation of the threonine biosynthesis-related gene may be controlled by the repressor and the promoter of the lambda phage. A plasmid containing the above- mentioned DNA is recommendably obtained by replacing the lambda phage PR promoter and the temperature sensitive C₁ repressor with the transcription- controlling region in the threonine operon of plasmid pVIC 40.;COPYRIGHT: (C)1993,JPO&Japio