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METHOD FOR OBTAINING PROTEIN HAVING RECOMBINANT DNA, RECOMBINANT VECTOR, CELL AND ACTIVITY OF N-CARBAMOYLSARCOSINEAMIDEHYDROLASE, SAID PROTEIN AND METHOD FOR MEASURING CREATININE
METHOD FOR OBTAINING PROTEIN HAVING RECOMBINANT DNA, RECOMBINANT VECTOR, CELL AND ACTIVITY OF N-CARBAMOYLSARCOSINEAMIDEHYDROLASE, SAID PROTEIN AND METHOD FOR MEASURING CREATININE
The invention relates to a recombinant DNA which contains, (1) the sequence depicted in SEQ ID NO: 1, (2) a sequence corresponding to this sequence within the scope of the degeneracy of the genetic code or (3) a sequence hybridising with a sequence from (1) and/or (2) under stringent hybridisation conditions, where the DNA sequence codes for a protein with N-carbamoylsarcosine amidohydrolase activity. The invention further relates to a recombinant vector with the DNA according to the invention and to a process for obtaining a recombinant protein with N-carbamoylsarcosine amidohydrolase activity and to the use thereof for determining creatinine.
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机译:本发明涉及一种重组DNA,其含有(1)SEQ ID NO:1所示的序列,(2)在遗传密码简并性范围内与该序列相对应的序列,或(3)与该序列杂交的序列。 (1)和/或(2)在严格杂交条件下的序列,其中DNA序列编码具有N-氨基甲酰基肌氨酸酰胺水解酶的蛋白质。本发明进一步涉及具有根据本发明的DNA的重组载体,以及用于获得具有N-氨基甲酰基肌氨酸酰胺水解酶活性的重组蛋白的方法及其在确定肌酐中的用途。
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