Procedure for the determination of the presence of substances important to the diagnosis, in particular Antibodies or antigens by Elisa using photometric Evaluation method

摘要

The present Invention refers to a procedure for the determination of the presence of liquid test substances important to the diagnosis, in particular Antibodies or antigens.Preferably by the Elisa method.Through photometric Evaluation of samples prepared from the liquid test Antibody / Antigen complexes Dyed and marked with EnzymesWhere Is Only One Test of the sample dilution, dilution test in this setting triggers a reaction of Antigen / AntibodyBeing a participant in the Reaction preferably attached to a Solid Surface, and being subjected to the complex of Antigen / Antibody reaction coloration afectuada enzymatically.To prepare a solution of coloration and from extinction as this solciu00f3n coloration, using Reference Data previously measured,Defines a title for the title of the final dilution is marked by the extinction of the solution of Coloring and the signal obtained from sedetermina analiticame adoNte Title according to the formula: Log Title Alpha.A (betaOJ equipra) which is the title of diluviu00f3n final obtainable using the dilution series title in formula being the reciprocal of the final dilution in which the signal is.The Signal Detection limit limit value in the front and drive to exhibition Witness Negative Values for Alpha and Beta with a Dilution of Rehearsals for the relevant conbinac establishedIon surface and immunologically reactive with anti immunoglobulin or Geno marked as detects Enzymes under the same reaction conditions and fixationPiloted through separate Series of dilutions of samples with known Title final Dilution of the analyte.