Method for the determination of the presence of diagnostically relevant substances, particularly antibodies or antigens, by the 'ELISA' method using photometric evaluation

摘要

is provided a method for the detection and quantification of stufenlo sen diagnostically relevant Substan zen, wherein a single sample approach to the study of the sample is sufficient. As a test, ELISA, RIA, nucleic acid hybridization, nephelometry methods can be used, among other things Bestimmungsme. As diagnostsich relevant substances z. As antigens, antibodies of the various classes of immunoglobulins, nucleic acids, or metabolic products of medical importance according rejected. Antibody complexes on a sample in the selected test dilution with dyed enzymatically labeled antigen / anti absorbance is measured, and from the obtained signal A _OD, the titer calculated according to the formula: lg titer = alpha. A determined, which can be equated to the obtainable by dilution series Endverdünnungstiter with good accuracy. The values ​​of alpha and beta are determined experimentally in separate series of experiments.