Thermally stable amylases and pullulanases from two anaerobic microorganisms

摘要

Amylases and pullulanases which can be obtained from Clostridium thermosaccharolyticum DSM 572 and Thermoanaerobacter ethanolicus DSM 2246 are described. The extracellular, thermally stable amylases and pullulanases are produced in a high concentration from the said thermophilic anaerobic microorganisms in a continuous process. These enzymes have a high stability at 60 DEG C and 70 DEG C in the absence of metal ions and substrate under aerobic conditions. The pH and the temperature optimum are in the same range, so that both enzymes can be employed together for starch hydrolysis.