New antibiotic Y1-HU3 has the following characteristics: (a) mol. wt. 469; (b) m.pt. 180-190oC, pale yellow crystals; (c) UV spectrum has two maxima at 312 nm and 300 nm (in chloroform); (d) soluble in methanol, chloroform; (e) insol. in n-hexane. The IR, 1H NMR and 13C NMR spectra are given. Antiobiotic Y1-HU3 is obtd. by culture of Pseudomonas species Y1-Hu (FERM-BP 1610). In an example Pseudomonas sp. Y1-HU is cultured in potato dextrose medium at pH and 28 deg. C for four days. The cell bodies are sepd. and the culture filtrate extracted with chloroform. The extract is concd. and chromatographed on silica gel (Wakogel C-200) using chloroform as eluent. The produce is dissolved in chloroform/methanol and hexane added to give antibiotic Y1-HU (10 mg from 1 litre culutre).
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机译：新的抗生素Y1-HU3具有以下特征：（a）摩尔。重量469; （b）点180-190oC，浅黄色晶体; （c）紫外光谱在312 nm和300 nm处有两个最大值（在氯仿中）; （d）易溶于甲醇，氯仿; （e）日晒。在正己烷中。给出了IR，1 H NMR和13 C NMR光谱。禁止使用抗生素Y1-HU3。通过假单胞菌属物种Y1-Hu（FERM-BP 1610）的培养。在一个例子中，假单胞菌属。 Y1-HU在马铃薯葡萄糖培养基中于pH和28度下培养。 C持续四天。细胞体是sepd。用氯仿萃取培养物滤液。提取物浓缩。并在硅胶（Wakogel C-200）上色谱分离，用氯仿作洗脱剂。将产物溶解在氯仿/甲醇中，并加入己烷，得到抗生素Y1-HU（从1升的凝块中提取10毫克）。
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