ON-LINE MULTIDIMENSIONAL CHROMATOGRAPHIC SYSTEM WITH LARGE VOLUME INJECTION HANDLING FOR SUPERCRITICAL FLUID CHROMATOGRAPHY

摘要

multidimensional chromatographic system line (10) and method combining a chromatography device (12) to a liquid chromatography device (58) of supercritical fluids. The system (10) includes an inlet column (36) which is deposited a fraction examine outgoing form eluted from the chromatography device (12) of liquid, means (54) interposed between the inlet column and the device chromatography (58) of supercritical fluid, for receiving and trapping on an impact surface (74) the analyte from the sinking fraction, and a valve arrangement (18, 20, 22, 24) for regulating fluid flow through the entire system (10). After the fraction to be examined has been deposited in the inlet column (36), the solvent of the liquid chromatography device is removed from the inlet column and the sinking fraction is extracted from the inlet column in a supercritical fluid flow. The flow of supercritical fluid is directed to the interface device (54) where the analyte is trapped while the supercritical fluid is decompressed and discharged. A subsequent supercritical fluid stream is then used to introduce the analyte trapped directly in the analytical column (56) of the chromatographic device (58) of supercritical fluids.