METHOD OF PEPTIDYLGLYCINE @@@-AMIDATING MONOOXYGENASE PURIFICATION AND A METHOD OF @@@-AMIDATED PEPTIDE PREPARATION

摘要

The use of biotechnology, medicine, biochemistry, obtaining medicines. SUMMARY OF THE INVENTION: An object of this invention to provide a method of purifying a -amidiruyuschey monooxygenase and a method for producing a -amidirovannogo pep- Chida. A peptidyl-glycine, -amidiruyu- conductive monooksidaza is an enzyme extracted from cancer cell lines and cancer tissues medullary having a molecular weight of about 60000-65000 Daltons. It is purified to such an extent that the use of electrophoresis cal procedures performed by a sodium dodecyl sulfate gels -poliakrilamid detects a single homogeneous well-defined band, and has a specific enzymatic activity not menee.50 mU per mg protein. Free or immobilized enzyme in presence of cupric ions, ascorbate and oxygen may be used for the preparation of a-amidated protein from polipeptidno- second substrate containing glycine radical with a terminal carboxyl group. The invention also encompasses a method pol; Scientists and -amidiruyuschego peptide of pep- tidnyh or polypeptide substrates containing terminal glycine residual group through a chemical reaction of the substrate with oxygen in the presence of purified enzyme beta askor- or copper. 1 yl. 00 of 00 kOe