METHOD OF PEPTIDYLGLYCINE @@@-AMIDATING MONOOXYGENASE PURIFICATION AND A METHOD OF @@@-AMIDATED PEPTIDE PREPARATION

摘要

use: u0431u0438u043eu0442u0435u0445u043du043eu043bu043eu0433u0438u00a0, medicine, u0431u0438u043eu0445u0438u043cu0438u00a0, obtaining drugs. the essence of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0: objects of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u044eu0442u0441u00a0 cleaning method and u0430u043cu0438u0434u0438u0440u0443u044eu0449u0435u0439 u043cu043eu043du043eu043eu043au0441u0438u0433u0435u043du0430u0437u044b and method u043fu043eu043bu0443u0447u0435u043du0438u00a0 and u0430u043cu0438u0434u0438u0440u043eu0432u0430u043du043du043eu0433u043e pep - chida.u043fu0435u043fu0442u0438u0434u0438u043b - u0433u043bu0438u0446u0438u043du043eu0432u0430u00a0 and, u0430u043cu0438u0434u0438u0440u0443u044e - u0449u0430u00a0 u043cu043eu043du043eu043eu043au0441u0438u0434u0430u0437u0430 u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 an enzyme u044du043au0441u0442u0440u0430u0433u0438u0440u0443u0435u043cu044bu0439 lines of cancer cells and tissues u043cu0435u0434u0443u043bu043bu00a0u0440u043du043eu0439 glands with u043cu043eu043bu0435u043a u0443u043bu00a0u0440u043du0443u044e mass of approximately 60000 - 65000 dalton.she is being treated, that the use of u044du043bu0435u043au0442u0440u043eu0444u043eu0440u0435 statistical procedures, u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u0435u043cu043eu0439 on u0433u0435u043bu00a0u0445 u0434u043eu0434u0435u0446u0438u043bu0441u0443u043bu044cu0444u0430u0442 u043du0430u0442u0440u0438u00a0 - polyacrylamide detects u0435u0434u0438u043du0443 yu u0433u043eu043cu043eu0433u0435u043du043du0443u044e well expressed by the band, and has a specific an enzymatic activity not u043cu0435u043du0435u0435.50 honey per mg of protein.free or u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu0439 enzyme in the presence of divalent ions of copper, u0430u0441u043au043eu0440u0431u0430u0442u0430 and oxygen can u0438u0441u043fu043eu043bu044cu0437u043eu0432u0430u0442u044cu0441u00a0u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 and u0430u043cu0438u0434u0438u0440u043eu0432u0430u043du043du043eu0433u043e protein from the u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u043du043e th substrate containing glycine radical with the angle u043au0430u0440u0431u043eu043au0441u0438u043bu044cu043du043eu0439 group.the invention also covers the method of half; u0443u0447u0435u043du0438u00a0 and u0430u043cu0438u0434u0438u0440u0443u044eu0449u0435u0433u043e u043fu0435u043fu0442u0438u0434u0430 of pep - u0442u0438u0434u043du044bu0445 or polypeptide substrates containing research u0433u043bu0438u0446u0438u043du043eu0432u0443u044e residual the group, through the chemical u0432u0437u0430u0438u043cu043eu0434u0435u0439u0441u0442u0432u0438u00a0 the substrate with oxygen in the presence of purified enzyme, u0430u0441u043au043eu0440 beta or copper. 1 il. 00 p.m. on ke