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Purification of pre - s by hbsag affinitaetbindung with polymerized serum albumin.
Purification of pre - s by hbsag affinitaetbindung with polymerized serum albumin.
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机译:hbsag affinitaetbindung和聚合血清白蛋白纯化前体蛋白。
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摘要
Recombinant pre-S-HBsAg is purified by a rapid and efficient two step chromatographic process. Yeast cells expressing recombinant pre-S-HBsAg are disrupted, the cell contents are clarified and separated by polymerized human serum albumin affinity chromatography. The pre-S-HBsAg is further purified by hydrophobic interaction chromatography using butyl agarose. This process results in pre-S-HBsAg that is greater than 90% pure.
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