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LEADER SEQUENCE FOR SECRETING HETEROLOGOUS POLYPEPTIDES IN YEAST, VECTORS USING IT, YEAST CELLS AND PROCESS FOR PRODUCING HETEROLOGOUS POLYPEPTIDES
LEADER SEQUENCE FOR SECRETING HETEROLOGOUS POLYPEPTIDES IN YEAST, VECTORS USING IT, YEAST CELLS AND PROCESS FOR PRODUCING HETEROLOGOUS POLYPEPTIDES
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机译:在酵母中分泌杂HE多肽的引子序列,使用它的载体,酵母细胞和生产杂LOGO多肽的方法
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摘要
A yeast expression cloning vector comprising the following sequence 5'-SP-Xn-3'-RS-5'-Xm-(NZT)p-Xq-PS-*gene*-3' wherein SP is a DNA sequence encoding a signal peptide, Xn is a DNA sequence encoding n amino acids, wherein n is 0 or an integer of from 1 to about 10 amino acids, RS is a restriction endonuclease recognition site provided at the junction of Xn and Xm, Xm is a DNA sequence encoding m amino acids, wherein m is 0 or an integer from 1 to about 10, (NZT)p is a DNA sequence encoding Asn-Xaa-Thr, wherein p is 0 or 1, Xq is a DNA sequence encoding q amino acids, wherein q is 0 or an integer from 1 to about 10, PS is a DNA sequence encoding a peptide defining a yeast processing site, and *gene* is a DNA sequence encoding a heterologous polypeptide. The vector may be used to construct synthetic leader peptide sequences by inserting random DNA fragments in the "RS" site, culturing a yeast cell transformed with this vector and screening the culture for secretion of the heterologous polypeptide.
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