METHOD FOR PRODUCTION OF THE DNA-SEQUENCES AND EXPRESSION VECTORS CONTAINING A KLUYVEROMYCES ALPHA-FACTOR LEADER SEQUENCE FOR DIRECTING SECRETION OF HETEROLOGOUS POLIPEPTIDES IN YEAST

摘要

DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces alpha -factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis alpha -factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.