Method and agent for detecting hydrogen peroxide or hydrogen peroxide forming system or peroxidase or peroxidative agent

摘要

The present invention is concerned with the use of aniline derivatives of the general formula: IMAGE (I) wherein R1 is a hydrogen atom or a lower alkyl or aryl radical, R2 is a hydrogen atom or a lower alkyl radical which can be substituted by hydroxyl, amino, carboxyl, lower alkoxycarbonyl, lower alkanoylamido, aryl lower alkyl or aryl or by a group of the structure: IMAGE in which R5 and R6 have the meanings given hereinafter, R3 is a hydrogen or halogen atom or a carboxyl group, R4 and R'4, which can be the same or different, are hydrogen or halogen atoms, carboxyl groups or lower alkoxy radicals or lower alkyl radicals which are preferably in the m-position and R1 and R4 or R2 and R4 or R4 and R'4, when the two substituents are ortho to one another, can together form a saturated or unsaturated hydrocarbon chain containing 2 to 6 carbon atoms which can be substituted by hydroxyl or oxo groups, R5 is a hydrogen atom or a lower alkyl radical and R6 is a hydroxyl group or a lower alkoxy, lower alkyl or aryl radical; and of the salts thereof with acids and bases (a) as colour former, together with an oxidisable coupling component, in the Trinder reaction for the detection of (a) hydrogen peroxide or hydrogen peroxide-forming systems or (b) peroxidase or peroxidate-acting substances or (b) together with an oxidation agent for the detection of aromatic amines or of systems forming such amines. The present invention also provides agents for the detection of redox systems containing the above aniline derivatives of general formula I. Furthermore, the present invention provides new aniline derivatives coming within the ambit of the above general formula I, as well as processes for the preparation thereof.