PURPOSE:To stably and efficiently produce shoot primordium by extracting shoot apex containing growth point of arbor plant from specific solution, transplanting into artificial solution medium containing inorganic salts and plant hormone and culturing with rotation under irradiation of light. CONSTITUTION:Shoot apex containing growth point of sterilely grown arbor plant is extracted from a solution containing polyvinyl pyrrolidone, then inorganic salts and plant hormone are added to the extract and transplanted into an artificial solution medium, thus cultured with rotation under irradiation of light to afford the aimed mass of shoot primordium. For instance, 1-(2- chloro-4-pyridyl)-3-phenolic urea of cytokinins is used as the plant hormone. As the arbor plant, evergreen broadleaf trees (e.g. eucalyptus), deciduous broadleaf trees (e.g. poplar), fruit trees (e.g. mandarin orange) and flowering trees and shrubs (e.g. rose) are exemplified.
展开▼