METHOD FOR INDUCING SEEDLING ANLAGE OF USEFUL PLANT AND METHOD FOR PROLIFERATING YOUNG SEEDLING FROM SAME INDUCED SEEDLING BASE AT LARGE AMOUNT

摘要

PURPOSE:To simply and efficiently induce a seedling anlage by subjecting an adventive embryo of an useful plant to rotation culture in a liquid culture medium under irradiation of light. CONSTITUTION:An explant (e.g. root, leaf, etc.) of plant is sterilized and then subjected to rotation culture in an adventive embryo inducing culture medium to provide the adventive embryo. The adventive embryo is cultured in a plant tissue culturing medium such as Murashige and Skoog medium to which as necessary a plant growth hormone (auxins of 0-100mg/l) are added under irradiation of light (having 5000-20000lux in the upper part of rotation and 100-5000lux in the lower part) in 0.5-10rpm numbers of revolution at 5-30 deg.C to provide the aimed seedling anlage. Furthermore, the seedling anlage is grown till the size of about 15mm and the grown seedling anlage is divided into 3-5mm diameter size and subjected to subculture to proliferate the seedling anlage in large quantities and the anlage is transplanted in a culture medium for growing into seedling to which a plant growth hormone is added and then subjected to stationary culture under irradiation of light having 1000-4000lux at 8-25 deg.C.