Prodn. of VP2 protein of African horse plague virus - by synthesising the DNA molecule codifying for the protein and expression in suitable host systems

摘要

The process consists of (a) obtaining a molecule of cDNA which codifies for the complete sequence of protein VP2, by designing specific oligo-nucleotides for the terminal regions of the VP2 gene and subsequently synthesising the cDNA molecule of complete length; and (b) obtaining recombinant VP2 proteins by expression of the cDNA molecule in suitable systems, e.g. baculovirus multiplied in insect cells.