Diagnostic and classification method for trypanosome cruzi species - uses synthetic oligo-nucleotide(s) for amplification of DNA fragments of the parasite

摘要

Using a sequence of nucleotides fragments of oligonucleotides with structure partially identical to those in the parasite can be synthesised. These fragments form probes which are used as bait for amplification of a larger DNA fragment of the parasite included between the synthetic oligonucleotides. Detection of the amplified DNA is direct evidence of the presence of the parasite in a sample. The sequence is used for detection of trypanosome cruzi by chain polymerisation reaction and for classification of the species and varieties. The nucleotide sequence is stated.