Retroviral mediated transfer of the human multiple drug resistance gene

摘要

This invention provides a mammalian retroviral producer cell constructed by transfecting a retroviral packaging cell with a retroviral vector containing the human multiple drug resistance gene. The mammalian retroviral producer cell produces retroviral particles suitable for transducing target cells. Accordingly, the producer cell of this invention can be used to transduce target mammalian cell with the human MDR gene, and with a second, non-selectable gene, e.g., insulin, beta -globin or a major histocompatibility gene. The producer cell of this invention can be used in methods of treating a mammal afflicted with a cancer and of treating a mammal afflicted with a disorder characterized by abnormal expression of a non-selectable gene which involve transducing suitable cells from the mammal with the human MDR gene and then selecting with an MDR-responsive drug for cells which express the MDR gene. This producer line is demonstrated to be safe and free of replication-competent retrovirus in contrast to the producer lines.