METHOD FOR PRODUCTION AND RECOVERY OF POLY-β-HYDROXYBUTYRATE FROM TRANSFORMED $i(ESCHERICHIA COLI)

摘要

Poly-β-hydroxybutyrate is produced by providing a culture Escherichia coli bacterial host cells transformed by a DNA sequence coding for the biosynthetic pathway of poly-β-hydroxybutyrate and a DNA sequence coding for the lysozyme gene; growing the culture and obtaining expression of the poly-β-hydroxybutyrate biosynthetic pathway and the lysozyme gene in each Escherichia coli bacterial host cell; lysing the Escherichia coli bacterial host cells and collecting the poly-β-hydroxybutyrate. An Escherichia coli HMS174(p4A [BstB], pLysS) deposited with the American Type Culture Collection under Accession No. 69001, comprising a plasmid containing a biosynthetic pathway coding for poly-β-hydroxybutyrate and a plasmid containing a lysozyme gene is disclosed.