首页> 外国专利> Method for the improved production and recovery of poly-amp;bgr;- hydroxybutyrate from transformed Escherichia coli

Method for the improved production and recovery of poly-amp;bgr;- hydroxybutyrate from transformed Escherichia coli

机译:改进转化大肠杆菌中聚-b-羟基丁酸酯的生产和回收的方法

摘要

Poly-&bgr;-hydroxybutyrate is produced by providing a culture of Escherichia coli bacterial host cells transformed by a DNA sequence coding for the biosynthetic pathway of poly-&bgr;-hydroxybutyrate and a DNA sequence coding for the lysozyme gene; growing the culture and obtaining expression of the poly-&bgr;-hydroxybutyrate biosynthetic pathway and the lysozyme gene in each Escherichia coli bacterial host cell; lysing the Escherichia coli bacterial host cells and collecting the poly-&bgr;-hydroxybutyrate. An Escherichia coli HMS174(p4A [BstB], pLysS) deposited with the American Type Culture Collection under Accession No. 69001, comprising a plasmid containing a biosynthetic pathway coding for poly-&bgr;-hydroxybutyrate and a plasmid containing a lysozyme gene is disclosed.
机译:聚-羟基丁酸是通过提供大肠杆菌细菌宿主细胞的培养物而产生的,该培养物由编码聚-羟基丁酸生物合成途径的DNA序列和编码溶菌酶基因的DNA序列转化而成。生长培养物并在每个大肠杆菌细菌宿主细胞中获得聚-b-羟基丁酸酯生物合成途径和溶菌酶基因的表达;裂解大肠杆菌细菌宿主细胞并收集聚-b-羟基丁酸酯。公开了以登录号69001保藏于美国典型培养物保藏中心的大肠杆菌HMS174(p4A [BstB],pLysS),其包含含有编码聚-b-羟基丁酸酯的生物合成途径的质粒和含有溶菌酶基因的质粒。

著录项

  • 公开/公告号US5512456A

    专利类型

  • 公开/公告日1996-04-30

    原文格式PDF

  • 申请/专利权人 JAMES MADISON UNIVERSITY;

    申请/专利号US19920890925

  • 发明设计人 DOUGLAS E. DENNIS;

    申请日1992-05-29

  • 分类号C12P21/06;C12P7/52;C12P1/04;

  • 国家 US

  • 入库时间 2022-08-22 03:38:40

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