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An improved method for the preparation of natural human growth hormone in pure form
An improved method for the preparation of natural human growth hormone in pure form
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机译:一种纯天然人生长激素的制备方法
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摘要
An improved method is described for the preparation of natural human growth hormone (hGH) in pure form, which comprises the preparation of a precursor of hGH by means of the culture of Bacillus subtilis cells transformed by a hybrid plasmid, the separation of the precursor from the total proteins, the digestion of the precursor with the enzyme Factor Xa and finally the purification of the natural hGH thus obtained from the enzymatic hydrolysis mixture. The growth hormone thus prepared has a purity of 100% or approximately 100% and is particularly useful for human treatment.
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