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A method for the production of foci - restriction endonuclease and - methylase.
A method for the production of foci - restriction endonuclease and - methylase.
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机译:一种产生病灶的限制性内切核酸酶和甲基化酶的方法。
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摘要
The present invention is directed to a method for cloning and producing the FokI restriction endonuclease by 1) introducing the restriction endonuclease gene from Flavobacterium okeanokoites IFO 12536 into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the FokI restriction endonuclease, and 3) purifying the FokI restriction endonuclease from the fermented host which contains the vector encoding and expressing the FokI restriction endonuclease activity.
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