A method for the production of acci - restriction endonuclease and - methylase.

摘要

The present invention is directd to a method for cloning and producing the AccI restriction endonuclease by (1) introducing the restriction endonuclease gene from into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the plasmid encoding and expressing the AccI restriction endonuclease activity, and (3) purifying the AccI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the AccI restriction endonuclease activity.