T4 endonuclease V DNA repair enzyme having specific activity at low salt concentrations

摘要

A T4 endonuclease V DNA repair enzyme contains an amino acid sequence within its carboxyl terminal region which is involved in dimer specific binding. The region includes polar nonaromatic basic amino acids and aromatic amino acids between amino acid 128 to 137 positions. The specific activity of the enzyme is greatly increased at low salt concentrations when substitutions are made in aromatic amino acids in the carboxy terminal region.