Purification of human interleukin-10 from a cell culture medium

摘要

A method for purifying Interleukin-10 (Il-10) from a eukaryotic cell culture medium. The method is comprised of subjecting IL- 10 containing cell culture medium to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.