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Selective detection of mycobacteria by nucleicacid probes derived from Mycobacterium kansasii
Selective detection of mycobacteria by nucleicacid probes derived from Mycobacterium kansasii
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机译:用来自堪萨斯分枝杆菌的核酸探针选择性检测分枝杆菌
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摘要
Oligonucleotide probes derived from Mycobacteria Kansasii capable of selectively hybridizing to Mycobacteria nucleic acid are disclosed. The oligonucleotide probe is selected from the group consisting of: (a) an oligonucleotide probe consisting essentially of the DNA sequence given herein as SEQ ID NO:1 (MK14); (b) oligonucleotide probes comprising fragments of MK14 which retain the capability of MK14 of selectively hybridizing to Mycobacteria nucleic acid; (c) oligonucleotide probes which hybridize to MK14 and are capable of selectively hybridizing to Mycobacteria nucleic acid; and (d) oligonucleotide probes which are complementary to any of the foregoing and are capable of selectively hybridizing to Mycobacteria nucleic acid. The probes are useful in nucleic acid amplification and hybridization assays for genus-specific detection of the mycobacteria, specific detection of M. kansasii and specific detection of the slow-growing mycobacteria. Methods of using the probes to detect and amplify Mycobacteria nucleic acid and kits containing the same are also disclosed.
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机译:公开了能够选择性地与分枝杆菌核酸杂交的源自堪萨斯分枝杆菌的寡核苷酸探针。寡核苷酸探针选自:(a)基本上由本文SEQ ID NO:1(MK14)给出的DNA序列组成的寡核苷酸探针; (b)包含MK14片段的寡核苷酸探针,该片段保留了MK14选择性地与分枝杆菌核酸杂交的能力; (c)与MK14杂交并能够与分枝杆菌核酸选择性杂交的寡核苷酸探针; (d)与前述任何一种互补并能与分枝杆菌核酸选择性杂交的寡核苷酸探针。该探针可用于核酸扩增和杂交测定中,用于分枝杆菌属特异性检测,堪萨斯分枝杆菌的特异性检测和缓慢生长的分枝杆菌的特异性检测。还公开了使用探针检测和扩增分枝杆菌核酸的方法以及包含该探针的试剂盒。
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