origonukureochido for enteritis vibrio germ detection and the detection method null which uses

摘要

PURPOSE:To enable simple and rapid detection with a high sensitivity by carrying out polymerase chain reaction(PCR) treatment using a specific oligonucleotide functioning as a primer for nucleic acid synthetic reaction. CONSTITUTION:Vibrio parahemolyticus is subjected to lytic treatment to provide a specimen (A) composed of a nucleic acid ingredient. The same sequence as the base sequence of tdh gene of the Vibrio parahemolyticus is chemically synthesized to afford a synthetic nucleotide (B) composed of a sequence group expressed by the formula. The ingredients (A) and (B) and a thermostable DNA polymerase are added to a buffer solution to afford a reaction solution (C). The resultant ingredient (C) is then thermally denatured at about 94 deg.C, subsequently annealed and further subjected to polymerization reaction. The PCR is performed in about 42 cycles to afford an amplified nucleotide fragment (D). The resultant ingredient (D) is then subjected to agarose electrophoresis, etc., to calculate the length of the nucleotide fragment and selectively detect the Vibrio parahemolyticus in the specimen.