ADSORPTION BODY FOR AFFINITY CHROMATOGRAPHY, SEPARATION METHOD FOR ANTIBODY USING THE SAME, MEASUREMENT METHOD FOR ANTIBODY CONCENTRATION, AND REFINERY CONCENTRATION METHOD FOR ANTIBODY PIECE

摘要

PURPOSE:To provide a method for separating and measuring only IgG of mouse or rat without being interfered by IgG of cow and for condensing an antibody piece with a high concentration. CONSTITUTION:Copper light chain connection protein of antibody is connected to a support as ligand and is used as an adsorption body for affinity chromatography. Using it, antibody is separated from a mixture by affinity chromatography. Also, the antibody concentration in cell incubation skim containing cow serum constituent is measured by a high-speed antibody chromatography. Further, an antibody piece with an antigen connection part is refined and condensed from a mixture containing a piece which is obtained by performing enzyme decomposition of antibody.