CODIFYING NUCLEOTIDIC SEQUENCE FOR A PROTEIN OF THE EXTERNAL MEMBRANE OF NEISSERIA MENINGITIDIS AND THE USE OF THAT PROTEIN IN PREPARING VACCINES.

摘要

Relates to the field of Biotechnology and Genetic EngineeringParticularly with a procedure for the isolation of a Nucleotide sequence that encodes a Protein of approximate Molecular Weight of64 000 Daltons (composition), located in the Outer membrane of Neisseria meningitidisAs well as the desoxiribonucleicos acid (DNA) obtained from her,Which are used for the transformation of a host organism.The Technical objective of the proposed solution is the identification of a Gene Sequence coding for a proteinA highly conserved and common for most pathogenic strains of Neisseria,With a view to obtain the Protein with a high degree of Purity and in commercially useful amounts via recombinantSo that it can be used in Diagnosis and Vaccine Preparation as an integral part of a Broad Spectrum immuneNoprotector.This Invention is based on the isolation of a New GE N encoding a Protein of Molecular Weight approximately 64 000Daltons (64 kDa) of the Outer membrane of Neisseria meningitidis B: 4: P1.15 and its Cloning and expression in prokaryotic host (E coli)It includes vehicles of expression containing sencuencia geneAs the microorganisms transformed with these vectors capable of producing Protein. !