STRAIN OF MICROORGANISM BREVIBACTERIUM FLAVUM CCM 4263 PRODUCING L-LYSINE

摘要

This patent describes L-lysine essential amino acid utilised, when fattening domestic animals (pigs) and this acid is created as a result of the synthesis and is accumulated in the media based on the mutant related to the bacterial family called Brevibacterium flavum CCM 4263, while this mutant is S-(2-aminoethyl)-L-cystein resistant, dependent of homoserine, leucine, valine and it is sensitive to sodium fluoropyruvate, which is being cultivated in flasks, however aerobic conditions are kept there and all this cultivation duration is 4 days at the temperature of 28 Celsius degrees, while saccharose, hydrolysate of pea flour and corn extract is present, and this mutant produced 55 g/l of L-lysine in the environment, however minimum of L-valine accumulation was observed (in g/l). The production of L-lysine, in the quantity of 92 g/l for 68 hours and conversion degree of 43 percent was reached, when used this strain in laboratory fermentation tanks.