IN VITRO METHOD FOR CIRCULAR SINGLE-STRANDED DNA

摘要

The invention relates to an in vitro method for preparing circular single-stranded DNA from circular double-stranded DNA comprising the steps of: (i) introducing a nick into a specific strand of the dsDNA; and (ii) removing the nicked strand to produce single-stranded DNA. The invention also provides a kit of components for use in the method of the invention.