USE OF ZWITTERIONS TO MOBILIZE ISOELECTRICALLY FOCUSED AMPHOLYTE ZONES

摘要

areas of normal of ampholytes, were stabilized by isoelectric focusing in a separation medium, such as a capillary tube, are provided through the introduction of a relatively high amount of effect in one of the electrode chambers.when a voltage is generated between the electrode chambers and during the concentration step, the zwitterion migrates into the separation medium according to its isoelectric point.the voltage is maintained to cause migration of the zwitterion in the capillary tube, which results in an enlargement area is concentrated areas, resulting from the concentration step and back in through the capillary tube to the axial a detection point or out of the capillary tube for recovery.