VACCINE CONTAINING LIVE VIRUSES FOR THERAPY OF VIRAL DISEASES AND MALIGNANCIES FOR THE TREATMENT OF VIRAL DISORDERS AND MALIGNANT TUMORS

摘要

The present invention relates to a method for purifying a virus comprising the step of purifying a liquid containing a virus by centrifugation, ultracentrifuging the supernatant to form a pellet, purifying the virus by sucrose gradient ultracentrifugation, rehydration and freeze-And a method for producing a vaccine. Preferably the denaturation is added as a protective colloid prior to lyophilization, with the starch being a hydroxyethyl starch of 100,000-300,000 molecular weight. The virus may be selected from the group consisting of avian infectious isovirus, avian herpesvirus, avian rotavirus, avian bronchitis, avian encephalitis, avian bursitis (gumbo) virus, magic viral virus, parvovirus, Human parvovirus, human adenovirus, and mixtures thereof. The purified virus vaccine produced by the above method is useful for the treatment and control of mammals suffering from viral diseases.