NUCLEIC ACID FRAGMENT ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, RECOMBINANT PLASMID DNA PPCL 4 ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, AND A METHOD OF NITRILE HYDRATASE PREPARING

摘要

FIELD: biotechnology, genetic engineering. SUBSTANCE: DNA fragment encoding polypeptide showing nitrile hydratase activity is prepared from the bacterial cells Pseudomonas chlororaphis B23. This nitrile hydratase shows capability to hydrate nitriles to amides. Recombinant DNA containing this gene and transformant formed by transformation of strain E. coli with recombinant DNA were prepared. Invention involves also a method of nitrile hydratase preparing using this transformant. EFFECT: preparing of DNA fragment indicated above. 5 cl, 10 dwg