Capillary electrophoresis using zwitterion-coated capillary tubes

摘要

A method and apparatus for controlling the velocity of electroosmotic flow (EOF) in high performance capillary electrophoresis (HPCE) are provided. The interior surface of the capillary tube is coated with a zwitterionic coating, such that the pH range within which EOF velocity may be controlled is broadened. The zwitterionic coating involves covalent, ionic or adsorptive binding of a zwitterionic species such as phosphoryl choline to the capillary wall.