METHOD FOR DISCRIMINATING TYPES OF ENTEROVIRUS 71 TYPE AND COXSACKIE A GROUP VIRUS 16 TYPE AND DNA PROBE AND DNA FRAGMENT USED THEREFOR

摘要

PURPOSE: To enable to discriminate the types of the Enterovirus 71 type (EV 71) and the Coxsackie A group virus 16 type (CA 16) in high accuracy. ;CONSTITUTION: The method for discriminating the types of the EV 71 and the CA 16 comprises (i) multiplying a DNA sequence coding for a part of the 5'-nontranslating regions of the EV 71 and the CA 16 and a part of Vp4 and Vp2 proteins having DNA sequences specific to the serum types of the viruses, (ii) determining the DNA sequence of the DNA region coding for the Vp4 protein in the multiplied DNA, (iii) designing DNA sequences complementary to the DNA sequences on the basis of DNA sequences specific to the serum types of the EV 71 and the CA 16 in the DNA sequences, respectively, and subsequently (iv) analyzing an ability for combining the obtained DNA probes with the multiplied DNA obtained by the method (i).;COPYRIGHT: (C)1996,JPO