Two-site immunoassay of antibody with chemiluminescent label and biotin-binding ligand

摘要

A method of detecting an antibody in a sample using a labelling compound and comprising the steps of mixing a ligand antigen, antibody or hapten bound to biotin with the sample; an antibody directed against the antibody to be detected bound to paramagnetic particles; and a chemiluminescent acridinium compound bound to avidin or streptavidin to form a solid phase complex; separating the solid phase from the liquid phase; and analysing the separated solid phase for the presence of chemiluminescent complex.