TWO-SITE IMMUNOASSAY FOR AN ANTIBODY WITH CHEMILUMINESCENT LABEL AND BIOTIN BOUND LIGAND Using Chemiluminescent Labels and Biotin-bound Ligands

摘要

The present invention relates to incomplete antigens bound to ligand antigens, antibodies or biotin; Antibodies against detected antibodies bound to paramagnetic particles; And a chemiluminescent acridinium compound bound to avidin, streptavidin, with the sample to produce a complex in which the solid phase is bound; Separating the solid phase from the liquid phase; A method for detecting an antibody in a sample using a chemiluminescent labeling compound, comprising analyzing the separated solid phase for the presence of the chemiluminescent complex.