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Two-site immunoassay for antibodies with chemiluminescent label and biotin-binding ligand
Two-site immunoassay for antibodies with chemiluminescent label and biotin-binding ligand
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机译:具有化学发光标记和生物素结合配体的抗体的两点免疫测定
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摘要
A method of detecting an antibody in a sample using a labelling compound and comprising the steps of mixing a ligand antigen, antibody or hapten bound to biotin with the sample; an antibody directed against the antibody to be detected bound to paramagnetic particles; and a chemiluminescent acridinium compound bound to avidin or streptavidin to form a solid phase complex; separating the solid phase from the liquid phase; and analysing the separated solid phase for the presence of chemiluminescent complex.
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