ALLERGEN CRY J II EPITOPE OF POLLEN OF JAPANESE CEDAR

摘要

PURPOSE: To obtain a new protein or peptide useful for diagnosing, preventing and treating pollinosis of Japanese cedar, containing a specific amino acid sequence and having at least one epitope of allergen of pollen of Japanese cedar, especially T cell epitope. ;CONSTITUTION: This protein or peptide contains the whole or part of an amino acid sequence including an amino acid sequence of the formula, has at least one epitope of allergen Cry-j-II of Japanese cedar, especially T cell epitope and is useful for diagnosing, preventing and treating pollinosis of Japanese cedar. The protein or peptide is obtained by passing whole RNA extracted from pollen of Japanese cedar by a conventional procedure through an oligo dT cellulose column to isolate mRNA, preparing a cDNA library using the mRNA, screening the library to select a cDNA coding for Cry-j-II, then incorporating the cDNA into a manifestation vector, transforming a host cell such as Escherichia coli and manifesting its gene.;COPYRIGHT: (C)1996,JPO