Procedure description of antibody repertoires (ab) and T cell receptor (TCR) of the immune system of an individual.

摘要

The present invention relates to a process of describing antibody repertoires (AB) AND cell receptors (TCR) IMMUNE SYSTEM OF INDIVIDUALS, wherein: A from a biological sample, TAKES THE OPPOSITE TRANSCRIPTION CONTAINING THE MARN; IS PERFORMED AFTER PRODUCT OF TRANSCRIPTION (or directly on the DNA extracted from the sample), about AMPLIFICATIONS separated by a PCR method TYPE FOR EACH PAIR OF BAIT V, C, where V corresponds to a variable segment Repertory CAUSE YC It hybridizes to the segment CONSTANT REPERTORIO STUDIED; ON EACH amplification products is performed, for each segment J MARKING THE REPERTORY, A elongation step BAIT used as an oligonucleotide specific to this SEGMENT JY amplification product as a template; FOR EACH elongation product corresponding to a triplet (V, C) J THUS OBTAINED APPEARS THE SIZE OF DIFFERENT extension products; THE DESCRIPTION OF THE RELEVANT REPERTOIRES ELEMENT FOR EACH WITH A TRIPLETE REPERTORIO VCJ and the size of the element.