DESCRIPTION PROCESS OF THE ANTIBODY (AB) REPERTOIRES AND OF THE T-CELL RECEPTORS (TCR) OF AN INDIVIDUAL'S IMMUNE SYSTEM

摘要

2100167 pctabs00130 9212260, the present invention relates to a method of description of the repertoires of antibody (ab) and t cell receptor (tcr) in the immune system of an individual.characterized in that: on the basis of a biological sample, we performed reverse transcription of the mrna that is then contained within it;the transcription product (or directly on dna extracted from the sample, separated by a method of amplification for each primer pcr (v, c)v corresponding to a segment register (variable and constant hybridizing segment repertoire studied; each of these pcr products was carried out.for each segment (in the inventory.an elongation step using specific oligonucleotide as primer for this segment i, and the amplification product as a template, each corresponding to a triplet of elongation (v.(c) thus obtained revealed the size of different products of elongation; description of directories corresponding to each element of the directory to a triplet and the size of the prcs.element.