DESCRIPTION PROCESS OF THE ANTIBODY (AB) REPERTOIRES AND OF THE T-CELL RECEPTORS (TCR) OF AN INDIVIDUAL'S IMMUNE SYSTEM

摘要

the present invention relates to a method of description of directoriesantibody (ab) and t cell receptor(crt) in the immune system of an individual, characterized in that: based ona biological sample is the transcript:production of mrna transcription is that it contains; the product oftranscript (or directly on dna extracted fromthe sample, pcr and separated by pcr methodeach pair of primer (v, c, v, corresponding toa variable segment and the register c hybridizing segmentcontinuous inventory on each of the products studied.amplification for each segment it is, in a directory.using the elongation step is: -in this segment i gonuclu00e9otide specific amplification product and the likematrix; equivalent for each product of elongationfor example, a triplet (v, c) thus obtained revealed the size ofvarious products of strain; the description of the effectsfor each element of the list has a triplet, prc,the size of the element.