RECOMBINANT THERMOSTABLE ENZYME WHICH RELEASES TREHALOSE FROM NON-REDUCING SACCHARIDE

摘要

Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6 - 6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85 .degree.C for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.