METHOD FOR THE CONSTRUCTION OF VECTORS FOR LACTIC ACID BACTERIA LIKE LACTOBACILLUS SUCH THAT THE BACTERIA CAN EFFICIENTLY EXPRESS, SECRETE AND DISPLAY PROTEINS AT THE SURFACE

摘要

The invention relates to a method to construct multi-purpose plasmid vectors that can be used for the introduction, stable maintenance and efficient expression and secretion of foreign genes or gene fragments in a variety of lactic acid strains such as Lactobacillus strains and Bifidobacterium, as well as to expression vectors to be used in this method. The invention also comprises a method of production of proteins and polypeptides heterologous and homologous to lactic acid bacteria at increased levels. Also comprised are lactic acid bacteria containing these expression vectors and producing these proteins or polypeptides.