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Method and reagent for enzymatic determination of creatinine
Method and reagent for enzymatic determination of creatinine
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机译:酶法测定肌酐的方法和试剂
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摘要
For the enzymatic determination of creatinine in aqueous solutions, the creatinine contained in the sample is converted by means of creatinine iminohydrolase into 1-methylhydantoin and a first molecule of NH3, the 1-methylhydantoin formed is converted by means of ATP-dependent 1-methylhydantoinase and N-carbamoylsarcosine amidohydrolase into sarcosine, CO2 and a second molecule of NH3, and the two molecules of NH3 formed are determined together.
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