Method for cryopreservation of primordial germ cells and germ cells

摘要

Disclosed is a method for cryopreservation of primordial germ cells and germ cells, which is characterized in that primordial germ cells or germ cells before meiosis that have been separated from embryos of birds are suspended in a freezing medium containing a cryoprotective agent and then frozen and stored therein. The method is employable for conservation of species of birds, for protection of rare species which are in danger of extinction and also for production of transgenic animals, production of novel gene marker animals on cell levels, production of disease model animals, production of useful substances by gene engineering and development of test methods for toxicity to germ-line cells.