HEGF VECTOR AND THE PREPARATION PROCESS OF HEGF

摘要

The invention is a method of preparing a new hEGF expression vector for mass production of hEGF. The expression vector is pTE105. The pTE105 is prepared by including cassette inserted with gene for coding hEGF between Omp leading sequence and universal translation termination in Omp A leading sequence - universal translation termination sequence - trp A transcription termination sequence. The cassette is made for control of translation by tac promoter. The tac promoter has two ribosome binding site, so the commencement of protein translation is accomplished effectively. The hEGF expression vector permits to produce hEGF continuosly and stably.