EXPRESSION VECTOR AND PROCESS FOR PRODUCING HUMAN EPIDERMAL GROWTH FACTOR (HEGF)

摘要

In a genetic engineering method, a large amount of hEGF was secreted in a medium of high density E. coli culture utilizing the controlled fed-batch system. The constructed plasmid in the recombinant host encoded a series of DNA sequences for a trip promotor, an E. coli alkaline phosphatase signal peptide and a synthetic human EGF. The invention provides hEGF in a naturally occurring form which is applicable to an antiulcer drug.