Purification Method of Human Interleukin-6 Expressed in Recombinant Escherichia Coli

摘要

The present invention relates to a method for purifying human interleukin-6 (IL-6) expressed in recombinant E. coli, the destruction of E. coli cells and recovery of insoluble precipitate, washing of insoluble precipitate using Triton X-100, Purification methods of the present invention, including dissolution of insoluble precipitate using Triton X-100 and guanidine salts, dialysis and recovery of soluble proteins in the presence of Triton X-100, and cation exchange chromatography, are simple and easy to implement IL-6 can be prepared.